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KMID : 1007520040130020244
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Food Science and Biotechnology
2004 Volume.13 No. 2 p.244 ~ p.247
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Molecular Cloning and Characterization of a Gene Encoding α-L-Arabinofuranosidase from Thermotoga maritima
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Yoon, Hyang-Sik
Keum, InKyung/Han, Nam Soo/Kim, Chung Ho
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Abstract
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A gene encoding α-L-arabinofuranosidase (α-L-AFase) was isolated from the hyperthermophilic microorganism Thermotoga maritima. The open reading frame (ORF) of α-L-AFase gene is 1,455 bp long and encodes 484 amino acid residues with a molecular weight of 55,265 Dz. The ORF of α-L-AFase gene was introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. The purified recombinant α-L-AFase showed a specific activity of 320 units/㎎ protein of p-nitrophenyl-α-L-arabinofuranoside, and showed the highest activity at 100-105℃ at pH5.5-6.0. The Km and Vmax values of the recombinant enzyme were 0.99 mM and 336.6 mmole·min^(-1) protein^(-1), respectively. L-Arabinose was released from oatspelts arabinoxylan by recombinant α-L-AFase, suggesting that L-arabinose could be produced from natural polysaccharides using the enzyme.
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